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Peer-reviewed veterinary case report

Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain.

Journal:
Veterinary research
Year:
2015
Authors:
Liu, Xinsheng et al.
Affiliation:
College of Food · United States

Abstract

The infectious dose of a virus pool of original US PEDV strain PC22A was determined in 4-day-old, cesarean-derived, colostrum-deprived (CDCD) piglets. The median pig diarrhea dose (PDD50) of the virus pool was determined as 7.35 log10 PDD50/mL, similar to the cell culture infectious titer, 7.75 log10 plaque-forming units (PFU)/mL. 100 PDD50 caused watery diarrhea in all conventional suckling piglets (n = 12) derived from a PEDV-naive sow, whereas 1000 and 10 000 PDD50 did not cause diarrhea in piglets derived from two PEDV-field exposed-recovered sows. This information is important for future PEDV challenge studies and validation of PEDV vaccines.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/26408019/