Peer-reviewed veterinary case report
Development and application of a novel histological multichrome technique for clam histopathology.
- Journal:
- Journal of invertebrate pathology
- Year:
- 2012
- Authors:
- Costa, Pedro M & Costa, Maria H
- Affiliation:
- IMAR - Instituto do Mar
Abstract
A novel and expedient histological tetrachrome technique was developed and applied to whole-body sections of the clam Ruditapes decussatus (L. 1758). The technique involves fixation in Carnoy's fluid followed by immediate embedding in paraffin with staining with a combination of Alcian Blue, Periodic Acid-Schiff's, Haematoxylin and Picric Acid. Fixation and staining was perfect for all tissues and resolved good identification of Perkinsus sp. infection and high structural detail. Among the surveyed fixatives, Bouin-Hollande's fluid also provided good results, however, fixation is potentially longer, polysaccharide staining was less intense and fibres appeared to be better preserved by Carnoy's.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22564259/