Peer-reviewed veterinary case report
Development and application of a qPCR assay forin Tibetan pigs.
- Journal:
- Frontiers in cellular and infection microbiology
- Year:
- 2025
- Authors:
- Xiao, Chendong et al.
- Affiliation:
- Center for Animal Disease Control and Prevention in Xiangcheng · China
Abstract
This study developed a rapid, sensitive, and specific TaqMan MGB based qPCR assay for detectingin Tibetan pigs by targeting thegene. The method showed no cross-reactivity with other common porcine enteric pathogens, and exhibited high sensitivity (detection limit: 7.056 copies/μL) and excellent repeatability (CV < 2%). A standard curve with good linearity (R² = 0.998) and 110.95% amplification efficiency was established. The assay was applied to 237 fecal samples collected from Tibetan pigs across eight townships in Xiangcheng County, Sichuan Province, in parallel with a national standard method. Results indicated a 19% overall positivity rate (45/237), with regional rates ranging from 11.11% to 26.67%. The established qPCR provides a reliable tool for field surveillance, and findings underscore the need to integratemonitoring into local prevention systems to support healthy Tibetan pig production.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/41416112/