Peer-reviewed veterinary case report
Development and application of a Saccharomyces cerevisiae-expressed nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibodies against infectious bronchitis virus.
- Journal:
- Journal of clinical microbiology
- Year:
- 2005
- Authors:
- Gibertoni, Aliandra M et al.
- Affiliation:
- Laborató · Brazil
Abstract
A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/15815038/