Peer-reviewed veterinary case report
Development and application of an indirect ELISA for the detection of antibodies to novel duck reovirus.
- Journal:
- Journal of virological methods
- Year:
- 2015
- Authors:
- Yun, Tao et al.
- Affiliation:
- Institute of Animal Husbandry and Veterinary Sciences · China
Plain-English summary
A new disease caused by a virus called novel duck reovirus (N-DRV) has been spreading among ducks in China since 2000. To help track this disease, researchers created a test that can detect antibodies to the virus in duck blood samples. This test, known as an indirect enzyme-linked immunosorbent assay (ELISA), was found to be very accurate, identifying 92.6% of true positive cases and 88.9% of true negatives when compared to another testing method. They tested over 1,200 duck blood samples and found that about 58% had antibodies against the virus, indicating exposure to N-DRV. Overall, this new test is effective and will help monitor and manage the spread of this virus in duck populations.
Abstract
A novel duck reovirus (N-DRV) disease emerged in China in 2000 and it has become an epidemic genotype. A test for detection of virus-specific antibodies in serum samples would be useful for epidemiological investigations. Currently, Currently, serological assays for N-DRV diagnosis are not available. A test for detection of virus-specific antibodies in serum samples would be useful for epidemiological investigations. In this study, a highly sensitive and specific indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to N-DRV was developed. The outer capsid (σC) of N-DRV was cloned and expressed in Escherichia coli as a coating antigen. The antigen concentration and serum dilution were optimized using a checkerboard titration. Furthermore, the specificity of σC-ELISA assay was confirmed by cross checking with other duck viral pathogens. In comparison with the western blot, the sensitivity and specificity of the σC-ELISA was 92.6% and 88.9%, respectively, and agreement of two tests was excellent with κ value of 0.786 (p < 0.05). A serological survey was performed using the assay on serum samples from different age and species of duck flocks in the Zhejiang and Jiangsu Province, China. The seropositive rate of the 1209 serum samples was 57.7%. In conclusion, the developed σC-ELISA assay is a very specific and sensitive test that will be useful for large-scale serological survey in N-DRV infection and monitoring antibodies titers against N-DRV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25907470/