Peer-reviewed veterinary case report
Test to detect Toxoplasma infection in dogs using rMAG1 antigen
By Zhuo, Xunhui et al.·Published in The Journal of parasitology·2017·View original on PubMed →
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Original publication title: Development and Application of an Indirect Enzyme-Linked Immunosorbent Assay Using Recombinant Mag1 for Serodiagnosis of Toxoplasma gondii In Dogs.
- Species:
- dog
Plain-English summary
A study found that a new blood test using a specific protein (rMAG1) can help detect Toxoplasma gondii infection in dogs more accurately than previous methods. Toxoplasma gondii is a parasite that can cause health issues in pets. The new test showed higher sensitivity and specificity, meaning it was better at identifying infected dogs without false positives. This could be a valuable tool for veterinarians to diagnose Toxoplasma infections in dogs, leading to better treatment options.
People also search for: dog Toxoplasma test · symptoms of Toxoplasma in dogs · how to treat Toxoplasma gondii in dogs
Abstract
Serologic tests are widely accepted and applied as means to detect anti- Toxoplasma gondii immunoglobulin G antibodies. In this study, recombinant matrix antigen (rMAG1) was induced by isopropyl-β-d-thiogalactoside and purified by nickel-nitrilotriacetic acid purification system. We then developed and optimized an indirect enzyme-linked immunosorbent assay (ELISA) through checkerboard assays using serial dilutions of antigens and sera to assess the potential use of rMAG1 in serologic detection of T. gondii infection in dogs. Serum samples from 93 domestic dogs were analyzed by western blot and rMAG1-ELISA. The results were compared with those obtained from an ELISA with the soluble Toxoplasma lysate antigens (TLA). We found that although yielding an excellent agreement (96.7%) with western blot data (κ = 0.9659), rMAG1-ELISA produced higher sensitivity (93.9% vs. 87.8%) and specificity (98.3% vs. 96.7%) than TLA-ELISA. In addition, receiver operating characteristic analysis also revealed that rMAG1-ELISA is in more agreement with western blot (area under the curve [AUC] = 0.985) relative to TLA-ELISA (AUC = 0.955). These results indicated that the rMAG1-ELISA established in this study provides a promising and reliable tool for serologic detection of T. gondii infection in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/28335675/