Peer-reviewed veterinary case report
Multiplex PCR test detects six viruses causing dog respiratory disease
By Piewbang, Chutchai et al.·Published in The Journal of veterinary medical science·2017·Department of Pathology·View original on PubMed →
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Original publication title: Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs.
- Species:
- dog
Plain-English summary
A group of dogs with respiratory illness were tested for several viruses that can cause coughing and breathing problems. Researchers developed a new test that can quickly identify multiple viruses at once, including canine influenza and canine distemper. This test was shown to be very accurate, detecting all six viruses in the samples taken from the dogs. The new testing method is helpful for veterinarians to diagnose respiratory infections more efficiently, using nasal or oral samples.
People also search for: dog coughing treatment · canine influenza virus symptoms · dog respiratory illness test
Abstract
Canine infectious respiratory disease complex (CIRDC) viruses have been detected in dogs with respiratory illness. Canine influenza virus (CIV), canine parainfluenza virus (CPIV), canine distemper virus (CDV), canine respiratory coronavirus (CRCoV), canine adenovirus type 2 (CAdV-2) and canine herpesvirus 1 (CaHV-1), are all associated with the CIRDC. To allow diagnosis, two conventional multiplex polymerase chain reactions (PCR) were developed to simultaneously identify four RNA and two DNA viruses associated with CIRDC. The two multiplex PCR assays were then validated on 102 respiratory samples collected from 51 dogs with respiratory illness by sensitivity and specificity determination in comparison to conventional simplex PCR and a rapid three-antigen test kit. All six viruses were detected in either individual or multiple infections. The developed multiplex PCR assays had a >87% sensitivity and 100% specificity compared to their simplex counterpart. Compared to the three-antigen test kit, the multiplex PCR assays yielded 100% sensitivity and more than 83% specificity for detection of CAdV-2 and CDV, but not for CIV. Therefore, the developed multiplex PCR modalities were able to simultaneously diagnose a panel of CIRDC viruses and facilitated specimen collection through being suitable for use of nasal or oral samples.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/27628592/