Peer-reviewed veterinary case report
Development and application of real-time PCR for detection of subgroup J avian leukosis virus.
- Journal:
- Journal of clinical microbiology
- Year:
- 2013
- Authors:
- Qin, Liting et al.
- Affiliation:
- Harbin Veterinary Research Institute · China
- Species:
- bird
Plain-English summary
This study focused on a virus called subgroup J avian leukosis virus (ALV-J), which affects birds and can lead to significant financial losses in poultry farming. Researchers developed a new testing method using real-time PCR, which is a technique that allows for quick and accurate detection of the virus in birds. This method can identify very small amounts of the virus and has proven to be reliable in tests. When examining tissue samples from birds suspected of having the virus, the new PCR method showed a positive result in about 60% of cases, compared to lower rates with other testing methods. Overall, the new real-time PCR test is a valuable tool for diagnosing ALV-J in birds.
Abstract
Subgroup J avian leukosis virus (ALV-J) is an avian retrovirus that causes severe economic losses in the poultry industry. The early identification and removal of virus-shedding birds are important to reduce the spread of congenital and contact infections. In this study, a TaqMan-based real-time PCR method for the rapid detection and quantification of ALV-J with proviral DNA was developed. This method exhibited a high specificity for ALV-J. Moreover, the detection limit was as low as 10 viral DNA copies. The coefficients of variation (CVs) of both interassay and intra-assay reproducibility were less than 1%. The growth curves of ALV-J in DF-1 cells were measured by real-time PCR, yielding a trend line similar to those determined by 50% tissue culture infective dose (TCID(50)) and p27 antigen detection. Tissue samples suspected of ALV infection were evaluated using real-time PCR, virus isolation, and routine PCR, and the positivity rates were 60.1%, 41.6% and 44.5%, respectively. Our data indicated that the real-time PCR method provides a sensitive, specific, and reproducible diagnostic tool for the identification and quantification of ALV-J for clinical diagnosis and in laboratory research.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23100340/