Peer-reviewed veterinary case report
Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle.
- Journal:
- Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
- Year:
- 2014
- Authors:
- Ramos, Carlos A N et al.
- Affiliation:
- Embrapa Gado de Corte Campo GrandeMS Brazil. · Brazil
Plain-English summary
Researchers have developed a new test to help detect antibodies against Anaplasma marginale, a disease that affects cattle. This test uses a simpler method called latex agglutination, which is less expensive and easier to use than the more complex enzyme-linked immunosorbent assay (ELISA). The new test showed a high level of accuracy, with 95% sensitivity (meaning it correctly identifies most infected cattle) and 92% specificity (meaning it correctly identifies most healthy cattle). This new test could be especially useful in areas where the disease is common but resources are limited. Overall, the latex agglutination test appears to be a promising alternative to the more expensive ELISA for diagnosing this disease in cattle.
Abstract
The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered important for the serological diagnosis of A. marginale, as they indicate that the test represents a rapid and low cost alternative to ELISA.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24948931/