Peer-reviewed veterinary case report
Development and evaluation of a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of type II porcine reproductive and respiratory syndrome virus.
- Journal:
- Journal of virological methods
- Year:
- 2012
- Authors:
- Gao, Ming et al.
- Affiliation:
- Department of Preventive Veterinary Medicine · China
Plain-English summary
This study focused on creating a new test to detect a specific virus called type II porcine reproductive and respiratory syndrome virus (PRRSV), which affects pigs. Researchers designed special tools called primers to help identify this virus in samples. They found that their new test, called RT-LAMP, was more sensitive than an older test method and worked well in identifying the virus even in mixed samples with other similar viruses. This means the RT-LAMP test can effectively tell if a pig is infected with type II PRRSV, which is important for managing pig health. Overall, the new test showed promising results in detecting the virus.
Abstract
The objective of this study was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of type II porcine reproductive and respiratory syndrome virus (PRRSV). Based on sequence alignment, four primers were designed amplifying the M gene of type II PRRSV and were subsequently utilized in an RT-LAMP assay. The RT-LAMP product had a ladder-like pattern of bands and the optimal reaction condition for this assay was determined to be 40 min at 63°C. Comparative analysis indicated that the RT-LAMP method was more sensitive than a conventional RT-PCR assay and comparable to a real-time PCR assay. In addition, the RT-LAMP assay was capable of detecting type II PRRSV in field samples and differentiating type II PRRSV from seven other porcine viruses which are all associated frequently with similar clinical symptoms.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22659065/