Peer-reviewed veterinary case report
Development and evaluation of a recombinant CP23 antigen-based ELISA for serodiagnosis of Cryptosporidium parvum.
- Journal:
- Experimental parasitology
- Year:
- 2009
- Authors:
- Wang, Chengmin et al.
- Affiliation:
- Institute of Zoology · China
Abstract
The CP23 gene of Cryptosporidium parvum strain isolated from Changchun in China was expressed in Escherichia coli BL21 strain and was purified as a recombinant protein. An indirect ELISA assay (CP23-ELISA) for antibody detection was established using the purified recombinant CP23 protein. Antigen coating conditions and serum dilution for the CP23-ELISA were optimized. The S/P ratio of the absorbency value was calculated in the CP23-ELISA to evaluate the serum antibody level of the field cow samples. It indicated that the CP23-ELISA assay, which was more convenient and easier to prepare than traditional methods, was a good candidate for evaluation of C. parvum exposure to domestic animal in field.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/19028493/