Peer-reviewed veterinary case report
Development and evaluation of enzyme-linked immunosorbent assay based on recombinant inorganic pyrophosphatase gene antigen for the detection of Mycoplasma suis antibodies.
- Journal:
- Research in veterinary science
- Year:
- 2012
- Authors:
- Liu, Jianzhu et al.
- Affiliation:
- College of Veterinary Medicine and Animal Science · China
Abstract
The recombinant ppa protein of Mycoplasma suis migrated to 21 kDa. Using this antigen, an ELISA system to detect the antibody against M. suis infection in swine was established. The rELISA demonstrated 98.5% specificities among negative samples and 96.9% sensitivity among positive samples with M. suis infection. A comparison of this ELISA system with an indirect hemagglutination assay (IHA) test using 132 swine samples revealed that the positive rate was 34.0% in ELISA and 28.0% in IHA. Compared with IHA, the present rELISA system using recombinant ppa antigen significantly improves the specificity, sensitivity, and stability for serodiagnosis of M. suis infection in swine.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21940024/