Peer-reviewed veterinary case report
How to test pigs for porcine parvovirus antibodies?
By Kong, Miaomiao et al.·Published in Journal of virological methods·2014·Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, China·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Development and evaluation of the rVP-ELISA for detection of antibodies against porcine parvovirus.
Plain-English summary
Researchers developed a new test called rVP-ELISA to detect antibodies against porcine parvovirus (PPV) in pigs. They created a version of a protein from the virus that closely resembles the natural one, which allowed them to set up this test. They tested 596 pig blood samples and found that about 87% were positive for PPV antibodies. When compared to another existing test, the new method showed a very high agreement rate of 96.7%. Overall, the rVP-ELISA is an effective and reliable way to identify PPV antibodies in pigs.
Abstract
The gene encoding the VP2 protein of porcine parvovirus (PPV) was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination (HA), Western blotting using PPV positive sera. The purified rVP2 proteins were used as coating antigen to establish a rVP-ELISA method for detection of PPV positive and negative sera from pigs. The optimal operating conditions of the rVP-ELISA were: the concentration of rVP2 proteins coated on the wells was 2 μg/mL; the diluted concentration of serum was 1: 150 and that of the enzyme-labeled antibody was 1: 6000. A total of 596 sera were detected by this assay, and the average positive rate was 87%. Compared with France LSI kit, the result showed that the coincidence rate was 96.7%. In conclusion, the rVP2-ELISA is a sensitive and specific method for detecting antibodies against PPV.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/24945904/