Peer-reviewed veterinary case report
Development and partial validation of a recombinant E2-based indirect ELISA for detection of specific IgM antibody responses against classical swine fever virus.
- Journal:
- Journal of virological methods
- Year:
- 2013
- Authors:
- Li, Wenliang et al.
- Affiliation:
- Institute of Veterinary Medicine · China
- Species:
- rabbit
Plain-English summary
This study focused on developing a new test to detect specific antibodies in pigs that indicate exposure to the classical swine fever virus, which is important for controlling the disease. The researchers created a test called the E2-IgM-ELISA, which showed that it could identify these antibodies effectively in pig blood samples. When they compared this new test to an existing one, they found that both tests agreed on results about 80% of the time. They also discovered that vaccinated piglets started showing these specific antibodies two weeks after vaccination, which was earlier than the other type of antibody they measured. Overall, the new E2-IgM-ELISA test works well for detecting these antibodies in pigs.
Abstract
Detecting classical swine fever virus specific antibody responses is critical for prevention and control of CSF. In this study, a ΔE2-based indirect ELISA was developed to detect specific IgM antibodies against CSFV. The optimized conditions that were determined experimentally are: a ΔE2 antigen concentration of 0.5 μg/ml, a serum sample dilution of 1/100 incubated at 37°C for 1.5 h, and a HRP conjugated rabbit anti-pig IgM dilution of 1/50,000 incubated at 37°C for 1 h. Three hundred clinical sera were tested with ΔE2-IgM-ELISA and IDEXX ELISA and the positive rates were 77.3% (232/300) and 71.7% (215/300), respectively. Concordance rate between them was 80.3% (241/300). The 59 inconsistent sera were tested further: among the 21 IDEXX ELISA +/ΔE2-IgM-ELISA - and 38 IDEXX ELISA +/ΔE2-IgM-ELISA - samples, 17 and 24 were determined positive by virus neutralization test; 15 and 25 were tested positive by ΔE2-IgG-ELISA, respectively. In addition, the E2-specific IgM antibody response in 15 vaccinated piglets could be detected 2 weeks post-vaccination and earlier than specific IgG antibody. It increased regularly and reached high levels by 6 weeks post-vaccination. The ΔE2-IgM-ELISA could be used for clinical detection and for exploring the kinetics of IgM antibody response.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23500647/