Peer-reviewed veterinary case report
Rapid test to detect feline herpesvirus type 1 antibodies
By Jianzhong Wang et al.·Published in Animal Diseases·2025·Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, GB·View original on DOAJ →
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Original publication title: Development and validation of a lateral flow immunoassay using colloidal gold for the identification of feline herpesvirus type 1
- Species:
- cat
Plain-English summary
A new test has been developed to quickly and accurately detect feline herpesvirus type 1 (FHV-1) in cats, which can cause serious respiratory and eye problems. This test works by identifying specific antibodies in a cat's blood and has shown to be very reliable, with a 97% sensitivity and 100% specificity. This means it can accurately tell if a cat has been infected with FHV-1, even when other viruses are present. The test is easy to use and remains effective for up to two years, making it a valuable tool for veterinarians in diagnosing and managing this common cat illness.
People also search for: cat respiratory infection test · feline herpesvirus symptoms · how to treat cat eye problems
Abstract
Abstract Feline herpesvirus type 1 (FHV-1) is a common and highly contagious pathogen in domestic cats that causes upper respiratory tract infections and ocular diseases. Accurate and rapid diagnosis of FHV-1 infections is essential for effective disease management and control. In this study, we developed an immunochromatographic lateral flow (ICLF) assay for the rapid and accurate detection of FHV-1-specific antibodies. The assay was founded upon the successful expression and purification of a 26 kDa recombinant glycoprotein B-glycoprotein D (gB-gD) fusion protein, which served as the primary antigen for the test. Rigorous testing for specificity and cross-reactivity confirmed the strip’s ability to exclusively detect FHV-1 antibodies, even in the presence of a variety of other feline viruses. The assay demonstrated excellent precision, reproducibility across dilutions, and long-term stability, retaining efficacy for 24 months during storage. Furthermore, clinical sample analysis revealed exceptional sensitivity (97%) and specificity (100%). In conclusion, the ICLF strip developed in this study represents a reliable, highly specific, and stable diagnostic tool for the rapid detection and management of FHV-1 infections in cats.
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Search related cases →Original publication on DOAJ: https://doi.org/10.1186/s44149-025-00170-1