Peer-reviewed veterinary case report
Test to detect COVID-19 and other respiratory infections in dogs
By Côme J. Thieulent et al.·Published in Viruses·2023·Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA, CH·View original on DOAJ →
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Original publication title: Development and Validation of a Panel of One-Step Four-Plex qPCR/RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Pathogens Associated with Canine Infectious Respiratory Disease Complex
- Species:
- dog
Plain-English summary
A study tested a new test that can quickly identify multiple germs causing respiratory problems in dogs, including the coronavirus (SARS-CoV-2). This is important because respiratory disease is common in dogs and can be caused by many different viruses and bacteria. The test was used on samples from dogs suspected of having these infections, and it found that the most common germs were Mycoplasma species and canine respiratory coronavirus. The new test can help veterinarians diagnose and treat respiratory illnesses more effectively, especially in cases where multiple infections are present.
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Abstract
Canine infectious respiratory disease complex (CIRDC) is the primary cause of respiratory disease in the canine population and is caused by a wide array of viruses and bacterial pathogens with coinfections being common. Since its recognition in late 2019, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been reported to cause respiratory disease in dogs. Therefore, the rapid detection and differentiation of SARS-CoV-2 from other common viral and bacterial agents is critical from a public health standpoint. Here, we developed and validated a panel of four one-step multiplex qPCR/RT-qPCR assays for the detection and identification of twelve pathogens associated with CIRDC (canine adenovirus-2, canine distemper virus, canine herpesvirus-1, canine influenza A virus, canine parainfluenza virus, canine pneumovirus, canine respiratory coronavirus, SARS-CoV-2, <i>Bordetella bronchiseptica</i>, <i>Streptococcus equi</i> subsp. <i>zooepidemicus</i>, <i>Mycoplasma cynos,</i> and <i>M. canis</i>), as well as the identification of three main CIV subtypes (i.e., H3N2, H3N8, and H1N1). All developed assays demonstrated high specificity and analytical sensitivity. This panel was used to test clinical specimens (<i>n</i> = 76) from CIRDC-suspected dogs. <i>M. canis</i>, <i>M. cynos</i>, and CRCoV were the most frequently identified pathogens (30.3%, 25.0%, and 19.7% of samples, respectively). The newly emerging pathogens CPnV and SARS-CoV-2 were detected in 5.3% of samples and coinfections were identified in 30.3%. This new multiplex qPCR/RT-qPCR panel is the most comprehensive panel developed thus far for identifying CIRDC pathogens, along with SARS-CoV-2.
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Search related cases →Original publication on DOAJ: https://doi.org/10.3390/v15091881