Peer-reviewed veterinary case report
Development of a blocking ELISA using a recombinant glycoprotein for the detection of antibodies to vesicular stomatitis New Jersey virus.
- Journal:
- Journal of virological methods
- Year:
- 2010
- Authors:
- Heo, Eun-Jeong et al.
- Affiliation:
- National Veterinary Research and Quarantine Service · South Korea
- Species:
- horse
Abstract
A recombinant glycoprotein (R-GP) of vesicular stomatitis New Jersey virus (VSV-NJ) was expressed in insect cells by a baculovirus system. Its utility as a diagnostic antigen in a blocking ELISA was investigated as an alternative to the current native GP extracted from VSV-NJ. With the cut-off value of 73% inhibition, the R-GP ELISA exhibited 99.1% specificity for naive sera from cattle and horses. It did not cross-react with VSV-Indiana (VSV-IN) positive sera and differentiated from foot-and-mouth disease and swine vesicular disease. Taken together, this is the first report that the R-GP has a potential to be used as a diagnostic antigen in place of the native GP for the detection of antibodies to VSV-NJ in cattle and horses.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/20018211/