Peer-reviewed veterinary case report
Development of a blocking latex agglutination test for the detection of antibodies to chicken anemia virus.
- Journal:
- Journal of virological methods
- Year:
- 2015
- Authors:
- Trinh, Dai Quang et al.
- Affiliation:
- Obihiro University of Agriculture and Veterinary Medicine · Japan
- Species:
- bird
Plain-English summary
Researchers developed a quick test to check for antibodies against chicken anemia virus (CAV) in chickens. They used special beads that stick to the virus and found that when they mixed these beads with infected chicken cells, they could see if the chickens had antibodies. The test was fast, giving results in just five minutes, and it worked well without confusing results from other viruses. When they tested 94 samples from chickens aged 17 to 63 weeks, the new test matched closely with a more complex method that is usually used, showing it is a reliable option for detecting CAV antibodies. Overall, this new test appears to be a useful and dependable tool for checking for CAV in chickens.
Abstract
A blocking latex agglutination test (b-LAT) developed in this study was evaluated for the detection of antibodies against chicken anemia virus (CAV) in chickens. Polystyrene latex beads were coupled with a neutralizing monoclonal antibody (mAb) to CAV (mAb-beads). When mAb-beads were mixed with antigens prepared from the lysate of MDCC-MSB1 cells infected with CAV, agglutination occurred. A short pre-incubation of CAV antigens with CAV-specific antiserum inhibited the agglutination of mAb-beads. The test results were obtained within 5min. The specificity of b-LAT was evaluated using sera from specific pathogen-free chickens and sera containing antibodies to avian influenza virus, Newcastle disease virus, infectious bursal disease virus, and Marek's disease virus; nonspecific agglutination and cross-reactivity with antibodies to unrelated viruses were not observed. The examination of 94 serum samples collected from commercial breeder chickens of various ages (17-63 weeks) revealed good agreement (93.6%, Kappa value=0.82) between b-LAT and a virus neutralization test, known to be most sensitive and specific in the detection of antibodies to CAV. These results indicate that b-LAT, a simple and rapid test, is a useful and reliable tool in CAV serology.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25952731/