Peer-reviewed veterinary case report
Development of a disperse dye immunoassay technique for detection of antibodies against Neospora caninum in cattle.
- Journal:
- The Korean journal of parasitology
- Year:
- 2013
- Authors:
- Selahi, Fatemeh et al.
- Affiliation:
- Islamic Azad University of Jahrom
Plain-English summary
This study looked at a new testing method to find antibodies against Neospora caninum, a parasite that can cause abortion in cattle. Researchers collected blood samples from 150 cows that had recently aborted and tested them using both a standard commercial test and a new in-house method. They found that about 34.6% of the samples tested positive with the new method, compared to 32% with the standard test, showing that both methods are quite similar in effectiveness. The new test is easier and cheaper to use, making it a good option for detecting this infection in cattle. Overall, the new testing method works well and could be a useful tool for farmers.
Abstract
In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (DDIA) compared to 32% obtained by ELISA kit. This study showed no significant difference between DDIA and ELISA. The results indicated that the DDIA provide an economic, simple, rapid and robust test for detection of N. caninum infection in cattle.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23467930/