Peer-reviewed veterinary case report
Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus.
- Journal:
- Virologica Sinica
- Year:
- 2022
- Authors:
- Zou, Tianli et al.
- Affiliation:
- Beijing Wantai Biopharmaceutical Co. · China
Abstract
• A probe-based insulated isothermal PCR (iiPCR) assay was developed for rapid and onsite detection of ASFV. • The developed iiPCR showed similar sensitivity and specificity with OIE recommended real-time PCR. • Blood samples could be directly applied as PCR template in iiPCR without DNA extraction.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/35257964/