Peer-reviewed veterinary case report
Development of a liquid-phase blocking ELISA based on foot-and-mouth disease virus empty capsid antigen for seromonitoring vaccinated animals.
- Journal:
- Archives of virology
- Year:
- 2013
- Authors:
- Basagoudanavar, S H et al.
- Affiliation:
- Indian Veterinary Research Institute · India
Plain-English summary
Researchers have developed a new test to help monitor whether cattle have developed immunity after being vaccinated against foot-and-mouth disease (FMD). This test uses a harmless version of the virus, created in the lab, instead of the inactivated virus that is currently used. They found that this new test performed very similarly to the traditional method, showing a strong correlation in results. This means that the new test could be a safe and effective way to check if vaccinated cattle have built up the right antibodies against FMD. Overall, the new test appears to work well for monitoring vaccination success.
Abstract
In foot-and-mouth disease (FMD) control programme, liquid-phase blocking ELISA (LPBE) is widely used to assay vaccine-induced seroconversion. Currently, the assay utilizes inactivated FMD virus antigen for the detection of antibodies in serum samples. To develop a non-infectious substitute for the antigen in LPBE, we expressed the structural polypeptide of FMDV (serotype A) using a baculovirus expression system, and show that inclusion of viral 3C with reduced protease activity resulted in a higher yield of structural proteins. Structural proteins expressed in insect cells assembled into empty virus-like particles (VLPs) and showed antigenicity comparable to chemically inactivated FMDV. Screening of serum samples from FMD-vaccinated cattle showed that the test performance of VLP-LPBE had a correlation of 0.89 with conventional inactivated virus antigen LPBE. The VLP-LPBE developed here demonstrates the diagnostic application of recombinant FMDV VLPs in monitoring seroconversion following FMD vaccination.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23242775/