Peer-reviewed veterinary case report
Development of a monoclonal blocking ELISA for the detection of antibody to Mycoplasma bovis in dairy cattle and comparison to detection by PCR.
- Journal:
- Veterinary immunology and immunopathology
- Year:
- 2005
- Authors:
- Ghadersohi, Ali et al.
- Affiliation:
- Department Microbiology and Immunology and Australian Institute of Tropical Veterinary and Animal Science James Cook University · Australia
Abstract
A monoclonal antibody blocking enzyme-linked immunosorbent assay (B-ELISA) was developed to detect antibodies to Mycoplasma bovis in cattle sera. The assay was highly specific and sensitive and there was no cross-reaction detected. This method revealed a high prevalence of antibodies (60%) to M. bovis in dairy cattle in North Queensland. The diagnostic potential of this B-ELISA for the detection of antibody to M. bovis was compared with its detection by PCR. There was a strong positive correlation between PCR and B-ELISA titers. Thus, the B-ELISA appears to be a valuable and reproducible tool in the serodiagnosis of M. bovis infection in cattle.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/15734539/