Development of a multiplex serological assay to detect immunoreactivity against a novelin military working dogs.

Abstract

pose a significant health risk to military working dogs (MWDs), and these zoonotic organisms may also cause disease in humans. According to the U.S. Army zoonotic disease surveillance program, there was a 47.4% seroprevalence forspp. among feral dogs in Iraq. This surveillance program identified infection with a novel speciesBartonella merieuxii (Bm) in 37% of the dogs by whole bloodDNA amplification and sequencing for theandgenes and intergenic spacer region. This bacterium has not been successfully cultured to date. The objectives of the present study were to (i) develop an assay to serologically detect infection withBm in dogs, and (ii) to define the prevalence andspecies acquired by MWD during deployment to Iraq. To establish a multiplex serological surveillance panel capable of assessing infection withspp., with special emphasis onBm, we identified20 peptides representing potential linear B cell epitopes. The newly developed serosurveillance panel was used to test paired samples (pre- and post-Iraq deployment) from 52 MWD. Conventionaldiagnostic testing (PCR and indirect immunofluorescence assays [IFA]) was also performed. Twenty-seven percent (14/52) of the paired samples had statistically significant antibody differences between pre- and post-Iraq deployment, suggesting seroconversion during deployment. In conclusion, the newly developed multiplex serologic assay, with its advantages of high throughput testing, requirement of low sample volumes, and ability to adapt newspecies to the panel, has the potential to serve as a new diagnostic tool and will be useful for serosurveillance.IMPORTANCEpose a significant health risk to military working dogs (MWDs), and these organisms also cause disease in humans.has not been assessed in MWD deployed to Iraq, despite a report that a novel,B. merieuxii (Bm), was detected in almost half of the stray dogs in Baghdad. Infection in dogs is similar to that in humans, and to date, at least three MWDs have died ofinfection. The goal of the proposed study is to develop an assay to serologically detect infection withBm in dogs and to define the prevalence andspecies acquired by MWD during deployment to Iraq. Dogs are potential reservoirs and sentinels for vector-borne infections and are implicated in the transmission of these infections to humans. Detecting the presence ofin Iraq-deployed MWDs is essential to identify and prevent the introduction of a newspecies to North America.