Peer-reviewed veterinary case report
Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease.
- Journal:
- Nephrology (Carlton, Vic.)
- Year:
- 2012
- Authors:
- Abbiss, Hayley et al.
- Affiliation:
- School of Natural Sciences Exercise · Australia
- Species:
- rodent
Abstract
AIM: The purpose of this research was to use metabolomics to investigate the cystic phenotype in the Lewis polycystic kidney rat. METHODS: Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups. RESULTS: With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model. CONCLUSION: The findings of this study demonstrate the potential of metabolomics to further investigate kidney disease.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22017187/