Peer-reviewed veterinary case report
Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection.
- Journal:
- Biotechnology letters
- Year:
- 2011
- Authors:
- Ren, Xiaofeng et al.
- Affiliation:
- Department of Preventive Veterinary Medicine · China
Plain-English summary
Researchers created a new test to detect a virus called porcine epidemic diarrhea virus (PEDV) in pigs. They took a specific part of the virus's genetic material and used it to produce a protein in a lab. This protein was then used to make a special antibody by immunizing rabbits. The tests showed that this antibody could identify cells infected with PEDV, while it did not react with other similar viruses. This means the new test is specific for detecting PEDV in pigs.
Abstract
A membrane (M), protein-based ELISA was developed to detect porcine epidemic diarrhea virus (PEDV). The M gene of PEDV was expressed in Escherichia coli. The purified recombinant M protein was used to immunize rabbits to generate a polyclonal antibody. Immunofluorescence analysis indicated that the anti-PEDV-M antibody reacted with PEDV-infected cells. The antibody was utilized to develop an indirect ELISA to detect PEDV. Other viruses, porcine transmissible gastroenteritis coronavirus, avian infectious bronchitis coronavirus, porcine reproductive and respiratory syndrome virus, classic swine fever virus and porcine pseudorabies virus, were unreactive.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/20882317/