Peer-reviewed veterinary case report
New quick antibody test detects Babesia gibsoni infection in dogs
By Verdida, R A et al.·Published in Parasitology·2005·Obihiro University of Agriculture and Veterinary Medicine, Japan·View original on PubMed →
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Original publication title: Development of a practical immunochromatographic test with recombinant P50 for the diagnosis of Babesia gibsoni infection in dogs.
- Species:
- dog
Plain-English summary
A group of dogs showing signs of anemia was tested for a blood infection caused by Babesia gibsoni, a parasite that can lead to serious health issues. Researchers developed a quick test that can detect antibodies to this parasite, and it proved to be accurate when compared to other established tests. The new test was able to identify infected dogs as early as six days after infection and showed a high level of agreement with the other testing methods. This means that veterinarians can use this new test to quickly and reliably diagnose Babesia gibsoni infections in dogs, helping them start treatment sooner.
People also search for: dog anemia symptoms · Babesia gibsoni test for dogs · quick test for dog blood infection
Abstract
An immunochromatographic test (ICT), using recombinant truncated P50 (P50t), for the detection of antibodies to Babesia gibsoni was developed and evaluated. Whereas all sera from specific pathogen-free dogs were clearly negative, all sera from dogs experimentally infected with B. gibsoni were clearly positive in the ICT. In addition, the ICT detected no cross-reactivity with sera from dogs experimentally infected with closely related parasites, B. canis canis, B. canis vogeli, and B. canis rossi, or with Neospora caninum, and Leishmania infantum. Sequential sera from a dog experimentally infected with B. gibsoni were tested with the ICT; it was shown that the specific antibodies are detectable as early as 6 days post-infection (p.i.) and that strong antibody responses remained until the end of the experiment (144 days p.i.). To evaluate the clinical application of the ICT, a total of 54 serum samples collected from domestic dogs that had been identified as having signs of anaemia at veterinary hospitals in Japan, were tested with the ICT, the previously established enzyme-linked immunosorbent assay (ELISA) and with the indirect fluorescent antibody test (IFAT). Twenty-four of the tested samples (44.4%) were positive in both ICT and ELISA, and (51.8%) in IFAT. The concordance between ELISA and ICT was found to be 100%, and 85.7% with IFAT. Taken together, the results above suggest that the ICT using P50t is rapid, simple, accurate, and suitable for use at clinical sites for the diagnosis of B. gibsoni infection in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/16336730/