Peer-reviewed veterinary case report
How real-time PCR detects canine respiratory coronavirus in dogs
By Mitchell, Judy A et al.·Published in Journal of virological methods·2009·Department of Pathology and Infectious Diseases, United Kingdom·View original on PubMed →
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Original publication title: Development of a quantitative real-time PCR for the detection of canine respiratory coronavirus.
- Species:
- dog
Plain-English summary
A group of dogs at a re-homing center with ongoing respiratory issues were tested for canine respiratory coronavirus (CRCoV), which can cause coughing and other breathing problems. Researchers developed a new test that accurately detects this virus in nasal and lung tissues. The results showed that CRCoV was most commonly found in the nasal passages and trachea of the affected dogs. This new test could help veterinarians diagnose CRCoV infections more effectively and understand how the virus spreads in dogs.
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Abstract
Canine respiratory coronavirus (CRCoV) has been detected recently in dogs with canine infectious respiratory disease and is involved in the clinical disease complex. CRCoV is a group 2 coronavirus most closely related to bovine coronavirus and human coronavirus OC43. A real-time PCR assay was developed for the detection of CRCoV. The assay was validated against cell culture grown virus and shown to have a high level of sensitivity. A range of tissue samples were collected from dogs at a re-homing centre with a history of endemic respiratory disease. The samples were tested using a conventional nested PCR assay and CRCoV was quantitated by real-time PCR. CRCoV was detected most frequently in the nasal mucosa, nasal tonsil and trachea. It was also detected in the lung, and bronchial lymph node. Of the enteric tissues, only one mesenteric lymph node sample was positive. In addition two colon samples were positive for CRCoV by nested PCR only. In conclusion, CRCoV appears to infect the upper respiratory tract preferentially. The CRCoV real-time PCR assay has proved to be a highly specific and sensitive assay that can be applied for diagnostic purposes as well as to investigate further the tissue tropism of CRCoV.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/19013196/