Peer-reviewed veterinary case report
Development of a real-time PCR assay using SYBR Green I for provirus load quantification in a murine model of AIDS.
- Journal:
- Journal of clinical microbiology
- Year:
- 2004
- Authors:
- Casabianca, Anna et al.
- Affiliation:
- Institute di Biological Chemistry Giorgio Fornaini · Italy
Abstract
A real-time PCR assay using SYBR Green I for quantification of provirus load in a murine model of AIDS (i.e., LP-BM5 infection) was developed and validated. In this method, data are normalized against the 18S rRNA gene. The method has a dynamic range of 8 logs and a sensitivity of one copy.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/15365045/