Peer-reviewed veterinary case report
Development of a real-time quantitative PCR for detecting duck hepatitis a virus genotype C.
- Journal:
- Journal of clinical microbiology
- Year:
- 2012
- Authors:
- Huang, Qiuxue et al.
- Affiliation:
- College of Life Science and Technology · China
Plain-English summary
Researchers have developed a new test to quickly detect duck hepatitis A virus genotype C (DHAV-C), which causes serious illness in ducks and has been leading to significant losses for duck farmers in China and South Korea. This test, called real-time PCR, can identify the virus in samples from infected ducks and can detect it as soon as 12 hours after infection, primarily in the liver. After 24 hours, the virus was found in several organs, including the heart, spleen, and lungs. This new method is important for diagnosing infections in ducks more rapidly and accurately. Overall, the test works well and could help improve the management of duck health.
Abstract
Recently, duck hepatitis A virus genotype C (DHAV-C), a causative agent of duck viral hepatitis, has been responsible for increasing economic losses in the duck industry in China and South Korea. In this study, a real-time PCR assay targeting the 2C gene for detecting DHAV-C was developed. The assay was confirmed to be specific and sensitive, and the minimum detection limit was 3.36 × 10(3) copies per reaction, making this assay suitable for rapid diagnosis of DHAV-C infection from clinical samples. In addition, the dynamics of the viral loads in tissues of specific-pathogen-free (SPF) ducklings infected with DHAV-C were investigated using this method. The DHAV-C could be detected earliest in the liver within 12 h postinfection. Moreover, high viral loads were identified in the heart, liver, spleen, lung, kidney, bursa of Fabricius, thymus, pancreas, brain, and small intestine after 24 h postinfection. Taking the data collectively, the study described in this report is the first to have developed a real-time PCR method for detection of DHAV-C and thus contributes to pathogenicity research.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22855514/