Development of a SYBR Green II Real-Time Polymerase Chain Reaction for the Clinical Detection of the Duck-Origin Goose Parvovirus in China.

Abstract

OBJECTIVE: To establish an efficient, convenient and quantitative method for the clinical detection of the duck-origin goose parvovirus. METHOD: In the present study, a real-time polymerase chain reaction (PCR) method was established for detecting the duck-origin goose parvovirus using the fluorescent chimeric dye SYBR Green II. Specific primers were designed to target a highly conserved region of the VP3 gene of the duck-origin goose parvovirus. RESULTS: This method was able to detect a minimum of 19.6 copies/μL of viral genomic DNA. Results showed that this method was faster and had a higher sensitivity than the traditional PCR in the clinical specimen test. In this paper, we developed a rapid, sensitive detection and quantitative analysis technology for the duck-origin goose parvovirus by real-time PCR assay. CONCLUSION: This test provides improved technical support for studies regarding the clinical diagnosis and epidemiological investigations of the duck-origin goose parvovirus.