Peer-reviewed veterinary case report
Development of a SYBR Green real-time PCR assay with melting curve analysis for simultaneous detection and differentiation of canine adenovirus type 1 and type 2.
- Journal:
- Journal of virological methods
- Year:
- 2015
- Authors:
- Balboni, Andrea et al.
- Affiliation:
- Department of Veterinary Medical Sciences · Italy
- Species:
- dog
Plain-English summary
Researchers have developed a new test that can quickly and accurately identify two types of canine adenovirus, which can cause serious illnesses in dogs. Canine adenovirus type 1 (CAdV-1) is linked to infectious canine hepatitis, while type 2 (CAdV-2) is associated with infectious tracheobronchitis. This new test is designed to work on samples from dogs and can tell the difference between the two types of viruses, which is important since dogs can be infected with both at the same time. The test has been shown to be very reliable and could be a helpful tool for veterinarians and labs to diagnose these infections more effectively. Overall, this new method could improve how we detect these viruses in dogs.
Abstract
Canine adenovirus type 1 (CAdV-1) and canine adenovirus type 2 (CAdV-2) cause infectious canine hepatitis (ICH) and infectious tracheobronchitis (ITB) in dogs, respectively. Cases of ICH have been documented in recent years and recent surveys have demonstrated a wide percentage of asymptomatic CAdV-1 infection in the canine population. Since both CAdV types are detectable in the same biological matrices, and viral coinfection with CAdV-1 and CAdV-2 are reported with high frequency, it is urgent to have available a rapid, highly sensitive and specific assay for the diagnosis of CAdV infection and distinction between CAdV-1 and CAdV-2. In order to detect canine adenovirus in biological samples and to rapidly distinguish the two viral types, a SYBR Green real-time PCR assay was optimized to discriminate CAdV-1 and CAdV-2 via a melting curve analysis. The developed assay showed high sensitivity and reproducibility and was highly efficient and specific in discriminating the two CAdV types. This reliable and rapid technique may represent a simple, useful and economic option for simultaneous CAdV types detection, which would be feasible and attractive for all diagnostic laboratories, both for clinical purposes and for epidemiological investigations.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26028428/