Peer-reviewed veterinary case report
How to detect encephalomyocarditis virus in pigs?
By Yuan, Wanzhe et al.·Published in Journal of virological methods·2014·College of Animal Medicine, China·View original on PubMed →
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Original publication title: Development of a TaqMan-based real-time reverse transcription polymerase chain reaction assay for the detection of encephalomyocarditis virus.
- Species:
- pig
Plain-English summary
This study focused on a virus called encephalomyocarditis virus (EMCV), which can cause serious heart issues in young pigs and reproductive problems in mother pigs. Researchers created a new test that can quickly and accurately detect this virus in samples. The test was found to be very sensitive, meaning it can identify even small amounts of the virus without getting confused by other viruses. The findings suggest that this new test will be useful for monitoring and diagnosing EMCV infections in pigs.
Abstract
Encephalomyocarditis virus (EMCV) is one of the major zoonosis pathogens and can cause acute myocarditis in young pigs or reproductive failure in sows. In this study, a TaqMan-based real-time reverse transcription polymerase chain reaction (RT-PCR) assay targeting 3D gene of EMCV was developed and their sensitivities and specificities were investigated. The results indicated that the standard curve had a wide dynamic range (10(1)-10(6) copies/μL) with a linear correlation (R(2)) of 0.996 between the cycle threshold (Ct) value and template concentration. The real-time RT-PCR assay is highly sensitive and able to detect 1.4×10(2) copies/μL of EMCV RNA, as no cross-reaction was observed with other viruses. These data suggested that the real-time RT-PCR assay developed in this study will be suitable for future surveillance and specific diagnosis of EMCV-infection.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/24996131/