Peer-reviewed veterinary case report
Rapid test strip for diagnosing canine babesiosis infection
By Wang, Jianzhong et al.·Published in Frontiers in veterinary science·2025·College of Veterinary Medicine, China·View original on PubMed →
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Original publication title: Development of an colloidal gold immunochromatography assay strip for the diagnosis of.
- Species:
- dog
Plain-English summary
A new test strip has been developed to quickly diagnose canine babesiosis, a serious tick-borne disease in dogs caused by the parasite Babesia canis. This test, which is easy to use and stable at room temperature, showed a sensitivity of 84% and a specificity of 93.6% when compared to traditional testing methods. It can help veterinarians identify infections more efficiently, especially in areas with limited resources. This means that if your dog is showing symptoms of babesiosis, such as fever or lethargy, this new test could provide a faster diagnosis and help guide treatment.
People also search for: dog fever symptoms · canine babesiosis treatment · tick-borne disease in dogs
Abstract
INTRODUCTION: Canine babesiosis, caused by Babesia canis, is a tick-borne hemolytic disease requiring rapid, reliable diagnostic tools to protect canine health in resource-limited settings. METHODS: We developed a colloidal gold immunochromatographic assay (CGIA) strip using a recombinant BcMSA1-BcSA1 fusion protein, expressed in Escherichia coli with a yield of 2.5 mg/L, combining hydrophilic domains of merozoite surface antigen (BcMSA1) and secreted antigen (BcSA1). Seventy-two serum samples from veterinary clinics in Shanxi Province, China (ethical approval SXAU-2022-013), were tested against a commercial ELISA kit (Anigen Rapid B. canis Ab Test Kit), with infections confirmed by microscopy and PCR. Specificity was assessed using sera positive for Theileria spp., Toxoplasma gondii, Ancylostoma caninum, Eimeria canis, Canine distemper virus, and Canine parvovirus. RESULTS: The CGIA strip showed no cross-reactivity, a detection limit of 1:8 for B. canis-positive sera, and retained efficacy after 18 months at room temperature. Sensitivity was 84%, specificity 93.6%, and Cohen's Kappa was 0.935 compared to ELISA. DISCUSSION: This stable, user-friendly CGIA strip offers an efficient point-of-care solution for B. canis detection, overcoming limitations of traditional methods and supporting epidemiological and clinical applications.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/40948622/