Peer-reviewed veterinary case report
Development of an indirect ELISA for the detection of duck circovirus infection in duck flocks.
- Journal:
- Veterinary microbiology
- Year:
- 2010
- Authors:
- Liu, Shao-Ning et al.
- Affiliation:
- Laboratory of Taishan Scholar Immunobiology · China
Abstract
Infection with duck circovirus (DuCV) is associated with growth retardation and developmental problems in farmed ducks. To detect DuCV-specific antibody in duck serum, an indirect enzyme-linked immunosorbent assay (iELISA) method was developed using the recombinant capsid protein antigen prepared by cloning the cap (Cap) gene of DuCV FJ0601 strain into pET-32a (+) vector and expressed in Escherichia coli. Using the optimized iELISA method, DuCV-specific antibodies were detected in 157 (12.96%) of 1211 samples obtained from 17 (89.47%) of 19 meat duck flocks aged from 25 to 40 days and in 89 (22.08%) of 403 samples obtained from 9 (75%) of 12 breeder flocks aged from 14 to 61 weeks. These results indicated that the iELISA method is useful for serological diagnosis of DuCV infection and epidemiological investigation.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/20409651/