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Peer-reviewed veterinary case report

Development of an SNP-based PCR assay for rapid differentiation of a Japanese live vaccine strain from field isolates of Erysipelothrix rhusiopathiae.

Journal:
Journal of microbiological methods
Year:
2015
Authors:
Shiraiwa, Kazumasa et al.
Affiliation:
National Institute of Animal Health · Japan

Abstract

The differentiation of vaccine from non-vaccine isolates is important for disease control. Based on single nucleotide polymorphisms identified by comparison of the genomes of the Koganei 65-0.15 vaccine strain and a reference strain of Erysipelothrix rhusiopathiae, we developed a PCR assay that can differentiate the vaccine strain from field isolates.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/26151408/