Peer-reviewed veterinary case report
Testing dog vaccine response to three Leptospira types
By Cariou, Carine et al.·Published in Veterinary immunology and immunopathology·2020·Boehringer Ingelheim, France·View original on PubMed →
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Original publication title: Development of antibody ELISA specific of Leptospira interrogans serovar Grippotyphosa, Canicola, and Icterohaemorrhagiae to monitor vaccine immunogenicity.
- Species:
- dog
Plain-English summary
A study found that after vaccination with a specific dog vaccine, 100% of dogs developed antibodies against the Leptospira bacteria Grippotyphosa and Icterohaemorrhagiae, while 45% showed antibodies against Canicola after the first dose. After a second dose, all dogs had antibodies for all three types. The new blood test developed in this study was found to be more sensitive than the traditional test, meaning it could detect more positive cases. This could help veterinarians better monitor how well the vaccine is working in dogs.
People also search for: dog leptospirosis vaccine effectiveness · dog vaccine antibody test · why is my dog getting vaccinated for leptospirosis
Abstract
The antibody response after primary vaccination and annual revaccination with a multivalent DAPPi-L vaccine was assessed respectively in SPF dogs and in client owned dogs against the Grippotyphosa (Lg), Canicola (Lc) and Icterohaemorrhagiae (Li) Leptospira serovars. To overcome limitations of the microscopic agglutination test (MAT), we developed serovar-specific and sensitive blocking ELISA assays. Serovar-specific antibodies against Lg, Lc and Li were detected in 100%, 45% and 91% of dogs, respectively, after the first dose of vaccine, and in 100% of dogs for all serovars after the second dose. In addition, mean ELISA antibody titers increased 14 days after annual revaccination with most dogs remaining ELISA antibody positive against Lg (85.3%), Lc (90%) and Li (100%). Parallel testing of sera from the annual revaccination study in the MAT and ELISA assays resulted in an overall agreement of 72%, 67%, 77% of samples for Lg, Lc and Li serovars, respectively. More sera tested positive by ELISA than by MAT, suggesting that the ELISA assay is more sensitive than the MAT. These three new antibody-based assays are the first suitable and reliable ELISA assays for the assessment of the canine antibody response following vaccination and an attractive alternative to the MAT.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31760236/