Peer-reviewed veterinary case report
Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD).
- Journal:
- Journal of veterinary science
- Year:
- 2012
- Authors:
- Jeong, Hyun-Jeong et al.
- Affiliation:
- Department of Infectious Diseases · South Korea
- Species:
- rodent
Abstract
Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP(res)) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23271186/