Peer-reviewed veterinary case report
Development of Nano-qPCR assay for the diagnosis of Infectious Bovine Rhinotracheitis Virus.
- Journal:
- Journal of virological methods
- Year:
- 2025
- Authors:
- Wang, Lin et al.
- Affiliation:
- College of Life Sciences · China
Plain-English summary
Infectious Bovine Rhinotracheitis Virus (IBRV) can cause serious breathing and reproductive issues in cattle, which can lead to big financial losses for farmers. Researchers created a new testing method called Nano-qPCR that can find IBRV more effectively than traditional tests. This new method was able to detect the virus in very small amounts and identified six cases that the older test missed. The Nano-qPCR test was confirmed to be accurate by isolating the virus from the samples. Overall, this new test is quick, affordable, and very sensitive, making it a valuable tool for monitoring and controlling this disease in cattle.
Abstract
IBRV causes severe respiratory and reproductive diseases in cattle, leading to significant economic losses. To improve diagnostic efficiency, this study developed a Nano-qPCR assay targeting the conserved UL50 gene of IBRV. The assay demonstrated high specificity, with no cross-reactivity to other tested bovine pathogens. Sensitivity testing demonstrated that Nano-qPCR could detect IBRV at concentrations as low as 3.5 × 10⁰ copies/μL, whereas conventional qPCR failed to detect it at this level. The assay was validated using clinical samples (nasal/oral swabs, feces, blood, and tissues) from unvaccinated cattle suspected of IBRV infection. Among 68 samples, Nano-qPCR detected six additional positive cases missed by conventional qPCR, which were later confirmed as IBRV-positive by viral isolation in MDBK cells. This confirmed the superior sensitivity of Nano-qPCR. In conclusion, the Nano-qPCR assay offers a rapid, cost-effective, and highly sensitive method for IBRV detection, making it a promising tool for disease surveillance and control in the cattle industry.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/40818494/