Peer-reviewed veterinary case report
Development of Organoids to Study Infectious Host Interactions.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2024
- Authors:
- Lahree, Aparajita & Gilbert, Leona
- Affiliation:
- Max Planck Institute of Molecular Biology and Genetics · Germany
Abstract
Emerging organoid research is paving way for studies in infectious diseases. Described here is a technique for the generation of stem-cell derived organoids for human small intestine and lung together with methods to infect such organoids with a mock pathogen (Cryptosporidium parvum). Such systems are amenable to imaging and processing for molecular biological analyses. It is the intent of this chapter to provide a simple, routine organoid procedure so that in vitro studies with Borrelia such as cell invasion and dissemination can be conducted.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/38165622/