Peer-reviewed veterinary case report
Development of polyclonal-antibody-coated immunomagnetic beads for separation and detection of koi herpesvirus in large-volume samples.
- Journal:
- Archives of virology
- Year:
- 2020
- Authors:
- Zhang, Min et al.
- Affiliation:
- The Institute of Animal Quarantine · China
Abstract
To separate and concentrate koi herpesvirus (KHV) from large-volume samples, a separation method based on immunomagnetic beads (IMBs) coated with polyclonal antibody directed against KHV was developed. After treatment with IMBs, viral DNA was extracted from samples and used as a template for quantitative PCR (qPCR). The results showed that the concentration of the template DNA extracted from the virus that had been separated using IMBs was 9.65-fold higher than that from virus not treated with IMBs. The detection limit of the IMBs/qPCR method was found to be at least 10 times lower than that of qPCR alone.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/32060793/