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Peer-reviewed veterinary case report

Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus.

Journal:
Virologica Sinica
Year:
2023
Authors:
Li, Yuchang et al.
Affiliation:
Institute of Microbiology and Epidemiology · China

Abstract

• Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection. • The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA. • No cross-reaction was found in 14 rash and fever-associated viruses. • The two RPA methods are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/36494078/