Peer-reviewed veterinary case report
Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus.
- Journal:
- Virology journal
- Year:
- 2017
- Authors:
- Yang, Yang et al.
- Affiliation:
- Lanzhou Veterinary Research Institute · China
Plain-English summary
Researchers have developed quick and effective tests to detect goatpox virus and sheeppox virus, which are important diseases affecting goats and sheep. These tests use a method called recombinase polymerase amplification (RPA) and can provide results in about 20 minutes. They specifically target a part of the virus's genetic material and have been shown to work well with samples from various organs and blood of infected animals. Importantly, these tests do not confuse the goatpox and sheeppox viruses with other similar viruses. Overall, this new testing method offers a fast and straightforward way to identify these viruses in livestock.
Abstract
BACKGROUND: Goatpox virus (GTPV) and sheeppox virus (SPPV), which belong to the Capripoxvirus (CaPV), are economically important pathogens of small ruminants. Therefore, a sensitive, specific and rapid diagnostic assay for detection of GTPV and SPPV is necessary to accurately and promptly control these diseases. METHODS: Recombinase polymerase amplification (RPA) assays combined with a real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the CaPV G-protein-coupled chemokine receptor (GPCR) gene, respectively. RESULTS: The sensitivity of both CaPV real-time RPA assay and CaPV RPA LFD assay were 3 × 10copies per reaction within 20 min at 38 °C. Both assays were highly specific for CaPV, with no cross-reactions with peste des petits ruminants virus, foot-and-mouth disease virus and Orf virus. The evaluation of the performance of these two assays with clinical sample (n = 107) showed that the CaPV real-time RPA assay and CaPV RPA LFD assay were able to specially detect SPPV or GTPV present in samples of ovine in liver, lung, kidney, spleen, skin and blood. CONCLUSIONS: This study provided a highly time-efficient and simple alternative for rapid detection of GTPV and SPPV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/28716095/