Peer-reviewed veterinary case report
Development of reverse transcription loop mediated isothermal amplification assay for rapid detection of bluetongue viruses.
- Journal:
- Journal of virological methods
- Year:
- 2015
- Authors:
- Mohandas, Sreekala S et al.
- Affiliation:
- Indian Veterinary Research Institute · India
Abstract
A single-step reverse transcription loop mediated isothermal amplification (RT-LAMP) assay targeting NS1 - a highly conserved gene among BTV serotypes was optimized and validated with seven serotypes: BTV-1, BTV-2, BTV-9, BTV-10, BTV-16, BTV-21 and BTV-23. The relative sensitivity of the assay was 0.3 TCID50 and no cross reactivity could be observed with foot and mouth disease, peste-des-petits-ruminants, goatpox, sheeppox and orf viruses. The established assay was also assessed by screening of clinical samples and the result is comparable with conventional RT-PCR. The RT-LAMP assay described here could be an additional tool to the existing assays for diagnosis/surveillance of BTV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26073661/