Peer-reviewed veterinary case report
Development of slide ELISA (SELISA) for detection of four poultry viral pathogens by direct heat fixation of viruses on glass slides.
- Journal:
- Journal of virological methods
- Year:
- 2014
- Authors:
- Desingu, P A et al.
- Affiliation:
- Indian Veterinary Research Institute · India
Plain-English summary
Researchers have created a new and simpler test called slide enzyme-linked immunosorbent assay (SELISA) to help diagnose four important viruses that can affect chickens: Newcastle disease virus, infectious bronchitis virus, infectious bursal disease virus, and egg drop syndrome 76 virus. This method involves heating the viruses on glass slides, which allows for easier detection of viral groups under a light microscope. This study is the first to report using heat fixation in this way for diagnosing these viral infections in poultry. The new test could make it easier for veterinarians to identify these viruses in chickens.
Abstract
The development of an easy and simpler method of slide enzyme-linked immunosorbent assay (SELISA) for the diagnosis of four economically important poultry viruses viz., Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious bursal disease virus (IBDV) and egg drop syndrome 76 virus (EDS 76) and the use of SELISA for semi quantitation of NDV are described. The positive signals for viral aggregates were detected under light microscope. This is the first report regarding the development of SELISA based on heat fixation for the diagnosis of viral pathogens.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25218174/