Peer-reviewed veterinary case report
Measuring a liver marker in dogs with chronic hepatitis using blood
By Lawrence, Yuri A et al.·Published in American journal of veterinary research·2019·View original on PubMed →
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Original publication title: Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for quantitative determination of trans-4-hydroxy-l-proline concentration in the serum of dogs with chronic hepatitis.
- Species:
- dog
Plain-English summary
A study found that dogs with chronic hepatitis had lower levels of a specific amino acid called trans-4-hydroxy-l-proline in their blood compared to healthy dogs. This was determined using a new testing method that accurately measures this amino acid in canine serum. The results showed that the median concentration of this amino acid was significantly lower in dogs with chronic hepatitis, which may indicate changes in how their liver processes nutrients. This information could help veterinarians better understand and manage liver disease in dogs.
People also search for: dog chronic hepatitis symptoms · low amino acid levels in dogs · liver disease in dogs treatment
Abstract
OBJECTIVE: To develop and analytically validate a liquid chromatography-tandem mass spectrometry method for measurement of endogenous trans-4-hydroxy-l-proline concentrations in canine serum and to assess serum trans-4-hydroxy-l-proline concentrations in dogs with chronic hepatitis. SAMPLE: Serum samples obtained from 20 dogs with histopathologically confirmed chronic hepatitis and 20 healthy control dogs. PROCEDURES: A liquid chromatography-tandem mass spectrometry method for quantification of trans-4-hydroxy-l-proline concentration was developed and assessed for analytic sensitivity, linearity, accuracy, precision, and reproducibility. Serum concentration of trans-4-hydroxy-l-proline in dogs with chronic hepatitis and healthy control dogs was measured. RESULTS: Observed-to-expected ratios for dilutional parallelism ranged from 72.7% to 111.5% (mean ± SD, 91.3 ± 19.6%). Intra-assay and interassay coefficients of variation ranged from 2.1% to 3.0% and 3.2% to 5.3%, respectively. Relative error ranged from -2.3% to 7.8%. Trans-4-hydroxy-l-proline concentrations were significantly lower in serum obtained from dogs with chronic hepatitis (median, 0.24 ng/mL; range, 0.06 to 1.84 ng/mL) than in serum obtained from healthy control dogs (median, 0.78 ng/mL; range, 0.14 to 4.90 ng/mL). CONCLUSIONS AND CLINICAL RELEVANCE: The method described here for the quantification of trans-4-hydroxy-l-proline concentration in canine serum was found to be sensitive, specific, precise, accurate, and reproducible. Dogs with chronic hepatitis had significantly lower serum trans-4-hydroxy-l-proline concentrations than did healthy control dogs, possibly as a result of altered hepatic metabolism of amino acids.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31034276/