Peer-reviewed veterinary case report
Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology.
- Journal:
- Scientific reports
- Year:
- 2019
- Authors:
- Peserico, Alessia et al.
- Affiliation:
- National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis · Italy
- Species:
- dog
Abstract
Prompt identification of the causative pathogen of an infectious disease is essential for the choice of treatment or preventive measures. In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after severe neurological signs were processed with the MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. Canine distemper virus (CDV) sequence reads were detected. Subsequently, a specific molecular test and immunohistochemistry were used to confirm the presence of CDV RNA and antigen, respectively, in tissues. This study supports the use of the NGS in veterinary clinical practice with potential advantages in terms of rapidity and broad-range of molecular diagnosis.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/30737428/