Peer-reviewed veterinary case report
How nanopore sequencing helps diagnose canine distemper virus
By Peserico, Alessia et al.·Published in Scientific reports·2019·National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Italy·View original on PubMed →
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Original publication title: Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology.
- Species:
- dog
Plain-English summary
A dog with severe neurological symptoms sadly passed away, and tests revealed it had canine distemper virus (CDV). Researchers used advanced sequencing technology to quickly identify the virus in the dog's brain tissue. They confirmed the presence of CDV using specific tests on the tissues. This case highlights how new testing methods can help veterinarians diagnose infectious diseases more rapidly, which is crucial for deciding on treatment and prevention strategies.
People also search for: dog neurological symptoms · canine distemper virus treatment · how to prevent dog distemper
Abstract
Prompt identification of the causative pathogen of an infectious disease is essential for the choice of treatment or preventive measures. In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after severe neurological signs were processed with the MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. Canine distemper virus (CDV) sequence reads were detected. Subsequently, a specific molecular test and immunohistochemistry were used to confirm the presence of CDV RNA and antigen, respectively, in tissues. This study supports the use of the NGS in veterinary clinical practice with potential advantages in terms of rapidity and broad-range of molecular diagnosis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/30737428/