Peer-reviewed veterinary case report
Diagnosis of botulism types C and D in cattle by a monoclonal antibody-based sandwich ELISA.
- Journal:
- The Veterinary record
- Year:
- 2011
- Authors:
- Brooks, C E et al.
- Affiliation:
- Agri-Food and Biosciences Institute Northern Ireland
Abstract
This study was undertaken to evaluate two monoclonal antibody-based sandwich ELISAs (sELISAs) for the detection of Clostridium botulinum neurotoxins (BoNTs) types C and D from culture-enriched intestinal content samples from cattle. To validate the diagnostic significance of the presence of cultivable, toxin-producing C botulinum in the intestines of cattle, samples from both suspect and non-suspect botulism cases were examined. BoNT was detected by both sELISAs in a greater number of suspect animals than by direct testing of uncultured samples by mouse bioassay. One sELISA detected two BoNT C and one BoNT Group III mosaic isoform in three animals that were missed by the other, and both sELISAs failed to identify samples from two mouse bioassay-positive BoNT C animals. BoNT D was also detected in one non-suspect sample by one of the sELISAs.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21493446/