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Peer-reviewed veterinary case report

Diagnosis of equid herpesviruses -1 and -4 by polymerase chain reaction.

Journal:
Equine veterinary journal
Year:
1992
Authors:
Sharma, P C et al.
Affiliation:
Department of Veterinary Pathology · United Kingdom
Species:
horse

Abstract

The polymerase chain reaction (PCR) is a sensitive technique used to detect DNA of viral pathogens. We have applied the technique to the detection of Equid herpesviruses-1 and -4 (EHV-1 and EHV-4) DNA within nasopharyngeal swab samples from horses. Ninety-eight samples from suspected field cases and in-contact horses were analysed. The assays were conducted blind and later decoded and compared with virus isolation data. Our results indicate that PCR is a sensitive and rapid technique for the diagnosis of EHV-1 and EHV-4 infection.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/1313360/