Peer-reviewed veterinary case report
Accuracy of rapid parvovirus test in diarrheic dogs with recent
By Kantere, Maria C et al.·Published in Journal of virological methods·2015·Department of Microbiology and Parasitology·View original on PubMed →
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Original publication title: Diagnostic performance of a rapid in-clinic test for the detection of Canine Parvovirus under different storage conditions and vaccination status.
- Species:
- dog
Plain-English summary
A group of young dogs with diarrhea was tested for canine parvovirus (CPV), a serious virus that can cause severe intestinal issues. Researchers compared a quick in-clinic test to a more reliable lab test (PCR) to see how well it worked. They found that while the quick test was very specific (meaning if it said positive, it was likely correct), it wasn't very sensitive, especially in vaccinated dogs. However, when the quick test was done right after collecting a sample, it performed much better. This means that if your dog has diarrhea and the quick test is positive, it likely has CPV, but a negative result doesn't rule it out, especially if your dog was recently vaccinated.
People also search for: dog diarrhea parvovirus test · young dog vomiting treatment · canine parvovirus symptoms and treatment
Abstract
Canine parvovirus (CPV) is one of the most common causes of acute haemorrhagic enteritis in young dogs, while clinical diagnosis is often indecisive. The aim of our study was to evaluate the diagnostic accuracy of an in-clinic rapid test in the detection of CPV infection in dogs. To this end, we compared the Rapid Diagnostic Kit of Canine Parvovirus, Coronavirus and Rotavirus antigen (Quicking(®)) to PCR, which is considered as the most reliable diagnostic method. A total of 78 duplicated faecal samples were collected from diarrhoeic dogs. Vaccination history within a month prior to the onset of diarrhoea was reported for 12 of the sampled dogs. The rapid diagnostic test was performed in 23 of the faecal samples directly, while the rest were placed into a sterile cotton tipped swab suitable for collection and transportation of viruses (Sigma Σ-VCM(®)) and stored at -20 °C. The sensitivity of the Quicking rapid diagnostic test compared to PCR in the total number of samples, in samples from non-vaccinated dogs and in samples tested directly after collection were 22.22% (95% CI: 13.27-33.57%), 26.67% (95% CI: 16.08-39.66%) and 76.47% (95% CI: 50.10-93.04%) respectively, while the specificity of the test was 100% in any case. In conclusion, negative results do not exclude parvoenteritis from the differential diagnosis, especially in dogs with early vaccination history, but a positive result almost certainly indicates CPV infection. An improved sensitivity may be expected when the test is performed immediately.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/25707551/