Peer-reviewed veterinary case report
Differentially methylated region in bovine MIMT1 detected by small-scale whole-genome methylation sequencing.
- Journal:
- Journal of applied genetics
- Year:
- 2019
- Authors:
- Rutkowska, Karolina et al.
- Affiliation:
- Institute of Genetics and Animal Breeding of the Polish Academy of Sciences
Abstract
We previously showed that deregulation of PEG3 domain genes is associated with intrauterine growth restriction (IUGR) in cattle. Here, we carried out genome-wide DNA methylation analysis of foetal placenta in two IUGR and wild-type samples and identified a differentially methylated region (DMR) in intron 2 of MIMT1. Pyrosequencing on a larger sample size (n = 20) confirmed significantly lower (P < 0.001) MIMT1-DMR methylation in foetal and maternal placenta of IUGR than wild-type conceptuses. Our study demonstrates that small-scale whole-genome bisulphite sequencing can be used to identify epigenetic regulatory elements in a defined genome locus.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/31468362/